GENOME SIZE ESTIMATION IN TWO POPULATIONS OF THE NORTHERN CHILEAN SCALLOP, ARGOPECTEN PURPURATUS, USING FLUORESCENCE IMAGE ANALYSIS

2005 ◽  
Vol 24 (1) ◽  
pp. 55-60 ◽  
Keyword(s):  
Image Analysis ◽  
Genome Size ◽  
Size Estimation ◽  
Fluorescence Image ◽  
Argopecten Purpuratus ◽  
Two Populations

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

Monitoring protease activity in human sputum samples by FRET-based probes via statistics-based fluorescence image analysis

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
F Stein ◽  
N Heath ◽  
S Dittrich ◽  
MA Mall ◽  
C Schultz
Keyword(s):  
Image Analysis ◽  
Protease Activity ◽  
Fluorescence Image

scholarly journals Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 563
Author(s):  
Monika Rewers ◽  
Iwona Jedrzejczyk ◽  
Agnieszka Rewicz ◽  
Anna Jakubska-Busse
Keyword(s):  
Genome Size ◽  
Species Identification ◽  
Dna Content ◽  
Size Estimation ◽  
Orchid Species ◽  
Infraspecific Taxon ◽  
2C Dna Content ◽  
Plant Families ◽  
Liparis Loeselii ◽  
Identification And Characterization

Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization.

Comparative genome size estimation of different life stages of grey mullet, Mugil cephalus Linnaeus, 1758 by flow cytometry

2021 ◽  
Author(s):  
Jani Angel J. Raymond ◽  
Mudagandur Shashi Shekhar ◽  
Vinaya Kumar Katneni ◽  
Ashok Kumar Jangham ◽  
Sudheesh Kommu Prabhudas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Mugil Cephalus ◽  
Life Stages ◽  
Size Estimation ◽  
Grey Mullet ◽  
Comparative Genome

scholarly journals Genome size estimation of Chinese cultured artemisia annua L.

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Zhixiang Liu ◽  
◽  
Shuai Guo ◽  
Jiang Xu ◽  
Yujun Zhang ◽  
...  
Keyword(s):  
Genome Size ◽  
Artemisia Annua ◽  
Size Estimation ◽  
Artemisia Annua L

scholarly journals Genome Size Estimation and Full-Length Transcriptome of Sphingonotus tsinlingensis: Genetic Background of a Drought-Adapted Grasshopper

2021 ◽  
Vol 12 ◽  
Author(s):  
Lu Zhao ◽  
Hang Wang ◽  
Ping Li ◽  
Kuo Sun ◽  
De-Long Guan ◽  
...  
Keyword(s):  
Genome Size ◽  
Genetic Background ◽  
Tandem Repeats ◽  
Full Length ◽  
Arid Environments ◽  
Size Estimation ◽  
Sequencing Data ◽  
Protein Coding ◽  
Grasshopper Species ◽  
Hsp Genes

Sphingonotus Fieber, 1852 (Orthoptera: Acrididae), is a grasshopper genus comprising approximately 170 species, all of which prefer dry environments such as deserts, steppes, and stony benchlands. In this study, we aimed to examine the adaptation of grasshopper species to arid environments. The genome size of Sphingonotus tsinlingensis was estimated using flow cytometry, and the first high-quality full-length transcriptome of this species was produced. The genome size of S. tsinlingensis is approximately 12.8 Gb. Based on 146.98 Gb of PacBio sequencing data, 221.47 Mb full-length transcripts were assembled. Among these, 88,693 non-redundant isoforms were identified with an N50 value of 2,726 bp, which was markedly longer than previous grasshopper transcriptome assemblies. In total, 48,502 protein-coding sequences were identified, and 37,569 were annotated using public gene function databases. Moreover, 36,488 simple tandem repeats, 12,765 long non-coding RNAs, and 414 transcription factors were identified. According to gene functions, 61 cytochrome P450 (CYP450) and 66 heat shock protein (HSP) genes, which may be associated with drought adaptation of S. tsinlingensis, were identified. We compared the transcriptomes of S. tsinlingensis and two other grasshopper species which were less tolerant to drought, namely Mongolotettix japonicus and Gomphocerus licenti. We observed the expression of CYP450 and HSP genes in S. tsinlingensis were higher. We produced the first full-length transcriptome of a Sphingonotus species that has an ultra-large genome. The assembly characteristics were better than those of all known grasshopper transcriptomes. This full-length transcriptome may thus be used to understand the genetic background and evolution of grasshoppers.

A new species of Acianthera (Pleurothallidinae, Orchidaceae) from Brazil

Phytotaxa ◽  
2019 ◽  
Vol 402 (1) ◽  
pp. 29
Author(s):  
JAN PONERT ◽  
ZUZANA CHUMOVÁ ◽  
TEREZIE MANDÁKOVÁ ◽  
PAVEL TRÁVNÍČEK
Keyword(s):  
New Species ◽  
Chromosome Number ◽  
Molecular Phylogeny ◽  
Genome Size ◽  
Size Estimation ◽  
Abaxial Side ◽  
Phylogenetic Placement ◽  
A New Species ◽  
Half Length

Acianthera sudae is newly described based on cultivated material from Brazil. It belongs to section Cryptophoranthae and differs from all others in this group by the larger flowers and sepals fused apically for more than half length, and a green-brown abaxial side of sepals with purple dots. Chromosome number (2n = 40) and genome size estimation (1C-value = 1.18 pg) is provided and its phylogenetic placement under the genus is supported with reconstruction of a molecular phylogeny using nuclear ribosomal ITS.

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